Glyoxalase Pathway Model from Martins et al 2001 |  PDF |
Catherine Lloyd (Bioengineering Institute, University of Auckland)
Table of Contents
In 2001, Margarida Martins et al published a paper which studied the kinetics of glyoxalase I and II in situ within Saccharomyces cerevisiae cells. The glyoxalase pathway is an enzymatic system which is widely distributed in living organisms (see Figure 1 below). One possible physiological function of the pathway may be the detoxification of the cytotoxin methylglyoxal, a by-product of metabolism. Another essential role of the glyoxalase pathway may be preventing the synthesis of advanced glycation end-products (AGE). These macromolecules are associated with diabetes mellitus and Alzheimer's disease.
For the reasons stated above, Margarida Martins et al believe it is important to understand how the glyoxalase system works in the detoxification of methylglyoxal in vivo. They addressed this problem by studying the kinetics of both enzymes in situ using permeabilised yeast cells.
The complete original paper reference is cited below:
In situ kinetic analysis of glyoxalase I and glyoxalase II in Saccharomyces cerevisiae, Margarida Martins, A. et al, 2001, Eur. J. Biochem, 268, 3930-3936. (The full text and PDF versions of the article are available to subscribers on the European Journal of Biochemistry's website.) PubMed ID: 11453985
The raw CellML description of the glyoxalase pathway model can be downloaded in various formats as described in the section “Download This Model”. For an example of a more complete documentation of another real reaction pathway, see The Bhalla Iyengar EGF Pathway Model, 1999.
In CellML, models are thought of as connected networks of discrete components. These components may correspond to physiologically separated regions or chemically distinct objects, or may be useful modelling abstractions. This pathway model has ten components representing chemically distinct objects (seven chemical species and three reactions). The CellML rendering of the glyoxalase pathway model is shown in Figure 2 (the different shapes in the diagram are explained in the notation guide). The action of a catalyst on a reaction is again represented by dashed lines.
glyoxalase_pathway_2001.xml — the raw XML.
glyoxalase_pathway_2001.html — an HTML version for browsing online.
glyoxalase_pathway_2001.pdf — a PDF version suitable for printing.
cellml_glyoxalase_pathway_2001.tar.gz — a gzipped tarball with the XML and this documentation.
glyoxalase_pathway_2001_maths.pdf — a PDF of the equations described in the model generated directly from the CellML description using the MathML Renderer.
